Combining insect genitalia preparation with DNA extraction

[Nieukerken, E.J. van]

Yes, I have done 10 th's this way, mostly of very small moths (Nepticulidae).
The protocol was developed by Sonja Knölke, Andreas Segerer and others in Munich. A paper (Knölke et al) is in press in Insect Systematics and Evolution, as far as I know not yet published. They suggest that this should be standard procedure! 
If you want the detailed protocol I think you should contact Andreas Andreas.Segerer at zsm.mwn.de 

The results are good: enough DNA extracted and perfectly clean genitalia!

Erik




Erik J. van Nieukerken

curator of Entomology (Microlepidoptera + Arachnida et al.)/ 
editor Tijdschrift voor Entomologie

National Museum of Natural History Naturalis
dep. of Entomology
PO Box 9517, 2300 RA Leiden, Netherlands

[street address: Darwinweg 2, 2333 CR Leiden]

direct phone: +31-71-56 87 682 (secretary ..622)
fax:      +31-71-5687666
e-mail:  nieukerken at nnm.nl
http://www.naturalis.nl/nieukerken
Tijdschrift voor Entomologie: http://www.nev.nl/tve


 -----Original Message-----
From:   Taxacom Discussion List [mailto:TAXACOM at LISTSERV.NHM.KU.EDU]  On Behalf Of Andrew Mitchell
Sent:   Thursday, December 09, 2004 2:38 AM
To:     TAXACOM at LISTSERV.NHM.KU.EDU
Subject:             Combining insect genitalia preparation with DNA extraction

Hello All,

Has anyone experimented with using a proteinase-K/buffer solution to clear insect abdomens for genitalia preparation, rather than using 10% KOH?  Seems to me this would allow you to extract DNA from the gunk that one normally throws out...  If you have tried this yourself or know of references please e-mail me.
Thanks,
Andrew
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Andrew Mitchell
Agricultural Scientific Collections Unit, OAI
NSW Department of Primary Industries
Forest Rd
Orange NSW 2800
AUSTRALIA

Tel: +61 (0)2 6391 3848
Fax: +61 (0)2 6391 3899
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