[Taxacom] Water soluble mounting media

Dan Lahr dlahr at ib.usp.br
Thu Mar 20 07:04:19 CDT 2014


thanks all for the tips!

__________________________________
Daniel J. G. Lahr
PhD, Assist. Prof.
Dept of Zoology, Univ. of Sao Paulo, Brazil
Office number: + 55 (11) 3091 0948
http://www.ib.usp.br/zoologia/lahr/



On Wed, Mar 19, 2014 at 5:12 PM, Tony Irwin <tony.irwin at btinternet.com>wrote:

> I note that a firm in Wales is offering what I assume to be PVOH/glycerine
> mountant for sale (25ml for £5.80 plus postage). See this link<http://www.ebay.co.uk/itm/Microscope-Slide-Mountant-Water-Based-L-C-25ml-/350077948977?pt=UK_BOI_Medical_Lab_Equipment_Lab_Supplies_ET&hash=item5182459431>.
> They describe it as being prepared to Lubkin & Carsten's classical
> formula.
> I was wondering whether anyone has information as to how long slides using
> this mountant will last, and how it compares in performance to DMHF.
> Tony
>
> Dr A.G.Irwin
> 47 The Avenues
> Norwich
> Norfolk NR2 3PH
> England
>
> mobile: +44(0)7880707834
> phone: +44(0)1603 453524
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>
> On 19 March 2014 16:04, Richard Zander <Richard.Zander at mobot.org> wrote:
>
>> Dan:
>>
>> Permanent? I don't know any permanent water-soluble mounting medium that
>> has the refractive index of glycerin. If the testae of the amoebas are
>> sturdy, you might try pure Elmer's clear glue. Use copiously, and it should
>> embed the specimen in polyvinyl alcohol.
>>
>> Richard
>>
>>
>> ____________________________
>> Richard H. Zander
>> Missouri Botanical Garden, PO Box 299, St. Louis, MO 63166-0299 USA
>> Web sites: http://www.mobot.org/plantscience/resbot/ and
>> http://www.mobot.org/plantscience/bfna/bfnamenu.htm
>> Evol. Syst.: <http://www.mobot.org/plantscience/resbot/EvSy/Intro.htm>
>> http://www.mobot.org/plantscience/resbot/EvSy/Intro.htm<
>> http://www.mobot.org/plantscience/resbot/EvSy/Intro.htmUPS>
>> UPS and FedExpr -  Missouri Botanical Garden, 4344 Shaw Blvd, St. Louis
>> MO 63110 USA
>>
>> ________________________________
>> From: daniel.lahr at gmail.com [mailto:daniel.lahr at gmail.com] On Behalf Of
>> Dan Lahr
>> Sent: Wednesday, March 19, 2014 10:35 AM
>> To: Richard Zander
>> Cc: taxacom at mailman.nhm.ku.edu
>> Subject: Re: [Taxacom] Water soluble mounting media
>>
>> Hi Richard,
>> Thank you for this link, I found the article quite helpful and will try a
>> couple of these.
>> I have been looking for a water soluble medium for permanent mounting of
>> testate amoeba slides.  Balsam (any kind) is a pain, and the classical
>> Euparal is quite hard to make and recently I have not been able to find all
>> ingredients.  Do you have any recommendations? I noticed you mention
>> glycerin-jelly as semi-permanent, but perhaps in appropriate controlled
>> environment?
>> Thanks,
>>
>> Dan
>>
>> __________________________________
>> Daniel J. G. Lahr
>> PhD, Assist. Prof.
>> Dept of Zoology, Univ. of Sao Paulo, Brazil
>> Office number: + 55 (11) 3091 0948
>> http://www.ib.usp.br/zoologia/lahr/
>> [http://www.ib.usp.br/webmail/assinatura.jpg]
>>
>>
>> On Sat, Mar 8, 2014 at 3:52 PM, Richard Zander <Richard.Zander at mobot.org
>> <mailto:Richard.Zander at mobot.org>> wrote:
>> Those who would like to try a new, simple, nonpoisonous, cheap, reliable
>> mounting medium for microslides might check out my paper in Phytoneuron:
>>
>> http://www.phytoneuron.net/2014Phytoneuron/32PhytoN-MicroMountingMedia.pdf
>> or
>> http://tinyurl.com/nh9lqfd
>>
>>
>> ____________________________
>> Richard H. Zander
>> Missouri Botanical Garden, PO Box 299, St. Louis, MO 63166-0299 USA
>> Web sites: http://www.mobot.org/plantscience/resbot/ and
>> http://www.mobot.org/plantscience/bfna/bfnamenu.htm
>> Evol. Syst.: <http://www.mobot.org/plantscience/resbot/EvSy/Intro.htm>
>> http://www.mobot.org/plantscience/resbot/EvSy/Intro.htm<
>> http://www.mobot.org/plantscience/resbot/EvSy/Intro.htmUPS>
>> UPS and FedExpr -  Missouri Botanical Garden, 4344 Shaw Blvd, St. Louis
>> MO 63110 USA
>>
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