[Taxacom] Propaganda (was: Molecules vs. Morphology)
Dr. David Campbell
amblema at bama.ua.edu
Mon Aug 17 10:58:03 CDT 2009
> It clearly demonstrates that a sloppy extraction can lead to totally
> inaccurate results (make sure you aren't extracting genetic material
> from the stomach or intestines).
It's not just "sloppy" extractions; sometimes the organism (or sample,
e.g. "it's already dead, so might as well try for DNA" with endangered
species) makes it difficult for you, and there are also plenty of
potential intermediate steps between tissue clip and extraction that
can potentially cause trouble. Possible contamination by bacteria is
especially difficult to prevent, since they're pretty much everywhere
that there are other organisms, plus places unsuitable for anything
else.
In general, more critical examination of molecular results would be
advisable. Not only are there the anomalies due to contamination,
misidentification, etc., but also just because your latest analysis
supports a clade does not mean that it is well-supported and the
definitive final answer. On the other hand, when a molecular clade is
unexpected but well-supported, preferably using more than one
analytical technique, it'd definitely worth going back and looking to
see if there are morphological, geographical, or other correlates.
--
Dr. David Campbell
425 Scientific Collections Building
Department of Biological Sciences
Biodiversity and Systematics
University of Alabama, Box 870345
Tuscaloosa AL 35487-0345 USA
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