photo manipulation

George (Buz) Wilson buz at MAIL.USYD.EDU.AU
Sat Mar 25 09:52:47 CST 2006


Whoops! I meant "terrestrial VERTEBRATE molecular biologist"
Apologies for the multiple posts
Buz Wilson
Australian Museum

> Don't forget that the Australian Museum is looking for two invertebrate
> systematists and one terrestrial ivertebrate molecular biologist. See
> the
> website http://www.austmus.gov.au/display.cfm?TypeName=Jobs
> Get your applications in the next week.
>
--
George (Buz) Wilson, Australian Museum
6 College Street, Sydney, NSW 2010  AUSTRALIA
Website:   http://www-personal.usyd.edu.au/~buz/home.html
EMAIL: buzw at austmus.gov.au
tel: 61 2 9320 6287        fax:  61 2 9320 6042


Quoting "George (Buz) Wilson" <buz at mail.usyd.edu.au>:

> I've gotten some nice colourised SEM effects (and useful ones, too) by
> capturing 3 identical images, but in three different capture modes: one
> in
> full secondary electrons, one in full backscatter electrons, and one
> that
> either emphasizes contrast or some other feature of the image, or just
> uses a blend of backscatter and secondary electrons. Load all three
> images
> in Photoshop and convert them to RGB colour channels. You can adjust the
> appearance of the image by choosing which image goes in which colour
> channel, and then modify later using the colour adjustments. You end up
> with an interesting colourised image this way, but also one that has
> details from all three images. I often find that converting this image
> back to greyscale for normal publication brings out some features that
> would be lacking in a single image capture.
>
> Folks can contact me offline if they want to know which photoshop
> thingies
> to use.
>

>
> --
> George (Buz) Wilson, Australian Museum
> 6 College Street, Sydney, NSW 2010  AUSTRALIA
> EMAIL: buz.wilson at austmus.gov.au
> tel: 61 2 9320 6287        fax:  61 2 9320 6042
>
>
> Quoting Paulo Petry <fishnwine at CHARTER.NET>:
>
> > Folks,
> >
> > You can use photoshop, transforming grayscale in rgb and them
> modulating
> > each color chanel and adjusting hue ans saturation. I do this to false
> > color x-rays and it works like a charm. In case of the x-ray, you have
> > to invert the image. Thus for aech gray scale image there might be a
> > different approach to render the best false coloring.
> >
> > Best Fishes,
> >
> > Paulo
> >
> >
> > ---- David Orlovich <david.orlovich at BOTANY.OTAGO.AC.NZ> wrote:
> > > Paul et al.
> > >
> > > Year's ago I used NIH Image to put false colours on SEMs.  It worked
> > > by allowing the use to change the colour table for the image, so
> that
> > > instead of going from black to white through the grey scales, it
> > > would allow you to go from (say) red to blue through the colours of
> > > the rainbow.  You could then scroll the colour table so that it
> would
> > > start (assign to black) any colour you want.  I think that will give
> > > you enough freedom to do what you want.  There were different colour
> > > tables to assign, so there were lots of pretty effects you could do.
> > >
> > > I see that you can still get NIH Image at the following URL (it's
> > > free, and it a Mac program, and also there is a Java version):
> > >
> > > http://rsb.info.nih.gov/nih-image/
> > >
> > > As I say, it was years ago (perhaps late 1980s - early 1990s) that I
> > > was doing this, but I'm sure it is still possible with that program
> > > and from memory it was easy.
> > >
> > > Cheers, David.
> > >
> > > On 25/03/2006, at 8:56 AM, Fabio Moretzsohn wrote:
> > >
> > > > Paul
> > > >
> > > > Some people color monochrome (grayscale) SEM or TEM photos in
> > > > Photoshop (or
> > > > similar) to emphasize a certain structure of interest, or just
> > > > because it
> > > > looks artistic. There are different ways to colorize a black and
> > white
> > > > photo, and maybe there are specialized software for this purpose.
> > > >
> > > > A researcher at the University of Hawaii, Tina Carvalho does some
> > nice
> > > > artistic colorizations of SEM and TEM micrographs colorized in
> > > > Photoshop:
> > > > http://www.pbrc.hawaii.edu/microangela/
> > > >
> > > > My two cents.
> > > >
> > > > Fabio
> > > >
> > > > -------------------------------------------------
> > > > Fabio Moretzsohn, Ph.D.
> > > > Post Doctoral Research Associate
> > > > Harte Research Institute of Gulf of Mexico Studies
> > > > Texas A&M University-Corpus Christi
> > > > 6300 Ocean Drive, Unit 5869
> > > > Corpus Christi, TX 78412-5869
> > > > Phone: (361) 825-3230
> > > > Fax: (361) 825-2050
> > > > fabio at falcon.tamucc.edu
> > > >
> > > >
> > > >
> > > > ----- Original Message -----
> > > > From: "Paul E. Hargraves" <pharg at GSO.URI.EDU>
> > > > To: <TAXACOM at LISTSERV.NHM.KU.EDU>
> > > > Sent: Friday, March 24, 2006 11:07 AM
> > > > Subject: [TAXACOM] photo manipulation
> > > >
> > > >
> > > >> Taxacomers:
> > > >> Changing the recent threads abruptly, I am wrestling with a
> problem
> > > >> I'm sure others have dealt with.  One often sees former
> black/white
> > > >> photos reproduced in various science magazines in blazing color,
> > esp.
> > > >> SEM & TEM photos.  I assume this is done with Photoshop in some
> > way.
> > > >> Can someone give details on how this is done?
> > > >> PEH
> > > >> --
> > > >> Paul E. Hargraves
> > > >> "Schau auf zu den Sternen, hab acht auf die Gassen" [-W. Raabe,
> > > >> 1831-1910]
> > > >> "Eamus, O Celtae; Eamus, O Tibialia rubentia" [anonymous, 64
> B.C.]
> > >
> > > Dr David Orlovich,
> > > Senior Lecturer in Botany.
> > >
> > > Department of Botany,
> > > University of Otago,
> > > P.O. Box 56,
> > > (Courier: 464 Great King Street)
> > > Dunedin,
> > > New Zealand.
> > >
> > > Phone: +643 479 9060
> > > Fax: +643 479 7583
> > > Mobile: +6421 122 7230
> > >
> > > Web: http://www.botany.otago.ac.nz/
> > > Ecology, Conservation and Biodiversity Research Group: http://
> > > www.otago.ac.nz/erg/
> > > Botanical Society of Otago: http://www.botany.otago.ac.nz/bso/
> > > Fungal Network of New Zealand: http://www.funnz.org.nz/
> >
>
>
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