DNA extraction from herbarium material

[Susan B. Farmer]

Back in the first of April, I had requested information on which protocols
that people used for extraction of DNA material from herbarium specimens.
I had several requests to post the results back to the list.  I apologize
for the tardiness of this reply -- it's the end of the semester crunch!

Anyway, I had 2 respondants use the DNeasy kit from Qiagen.
I had 2 respondants use the protocol from Lookerman and Jansen in Sampling the
        Green World.
1 used a modified CTAB protocol which is what my Major Professor normally
        uses as well.
I also found a protocol in the taxacom archives ...
        http://www.keill.ukans.edu/~gophtax/_gophtax.feb.97/0094.html
        This protocol does not use CTAB.
I also know that the PureGene DNA extraction kit claims that it can be used
        with dried material -- but I do not know if this is "fresh dried"
        or if this will work with older herbarium material.
My personal favorite, though, involved sun-dried tomotoes, garlic, and
        a light, white, wine ...

I hope this information helps others.

Susan
-----
Susan Farmer
sfarmer at goldsword.com
Botany Department, University of Tennessee
http://www.goldsword.com/sfarmer/Trillium