handling parasitic nematodes

[Luis C. Muniz]

Dear Marion,

It=B4s really a good problem to work with helminths preserved in formalin
many years ago, the best way is put it in a solution of glycerine (+ - 20%)
to try to make it more soften.
I don=B4t know how you prepare posteriorly the nematoids, but here in our la=
b
we mount the helminths (not definitely), only to examine before to mount in
balsam, in a slide with phenol and posteriorly in a acethic acid, this
procedure make the helminth more clear.


Dr Luis C. Muniz
Substitute Curator
FIOCRUZ
Instituto Oswaldo Cruz
Depto de Helmintologia
Lab Helmintos Parasitos de Vertebrados
Cole=E7=E3o Helmintol=F3gica do Instituto Oswaldo Cruz
Av. Brasil 4365
Rio de Janeiro - RJ
CEP 21045-900
BRASIL=20

At 18:08 23/02/99 +0100, you wrote:
>Dear taxacomers,
>Does anybody have experiences in handling parasitic nematodes (Strongylida)
>which were collected 30 years ago? I have the following questions:
>
>Is there any disadvantage in transferring specimens from formalin (in which
>they were stored for 30 years) into ethanol 70-80% with 1% glycerine? Would
>you know of any method to soften specimens so that they can be manipulated
>more easily for microscopic slides?
>
>
>----------------------------------------
>Marion Link
>Museum fuer Naturkunde
>Institut fuer Systematische Zoologie
>Invalidenstr. 43; 10115 Berlin; Germany
>Tel. +49/30/20938525
>Fax. +49/30/20938528
>
>